Table 1.
Primer sequences for real-time polymerase chain reaction
| Group | Gene name | Sequence | |
|---|---|---|---|
| T cells | CD3 | Forward | GATGCAGTCGGGCACTCACT |
| Reverse | CATTACCATCTTGCCCCCAA | ||
| Helper T cells | CD4 | Forward | GCCAACCCAAGTGACTCTGT |
| Reverse | TCTCCTGGACCACTCCATTC | ||
| Cytotoxic T cells | CD8 | Forward | ACTTGTGGGGTCCTTCTCCT |
| Reverse | GTCTCCCGATTTGACCACAG | ||
| NK cells | CD94 | Forward | GAGCCAGCATTTACTCCAGGAC |
| Reverse | GCACAGAGATGCCGACTTTCGT | ||
| B cells | B220 | Forward | ACA GCC AGC ACC TTT CCT AC |
| Reverse | GTG CAG GTA AGG CAG CAG A | ||
| CD19 | Forward | AAGGGGCCTAAGTCATTGCT | |
| Reverse | CAGCAGCCAGTGCCATAGTA | ||
| Myeloid cells | CD11b | Forward | CAGCCTTTGACCTTATGTCATGG |
| Reverse | CCTGTGCTGTAGTCGCACT | ||
| CD68 | Forward | GCTACATGGCGGTGGAGTACAA | |
| Reverse | ATGATGAGAGGCAGCAAGATGG | ||
| Oncogene | c-Myc | Forward | ACCACCAGCAGCGACTCTGA |
| Reverse | TGCCTCTTCTCCACAGACACC | ||
| Tumor suppressor | P21 | Forward | GGAAGACCATGTGGACCTGT |
| Reverse | GGCGTTTGGAGTGGTAGAAA | ||
| Tumor suppressor | P53 | Forward | AGGCCTTGGAACTCAAGGAT |
| Reverse | TGAGTCAGGCCCTTCTGTCT | ||
| Senescence | P16 | Forward | TGAGCACTCACGCCCTAAGC |
| Reverse | TAGCAGTGTGACTCAAGAGAAGCC | ||
| Housekeeping | β-Actin | Forward | TGAAGTGTGACGTGGACATC |
| Reverse | GGAGGAGCAATGATCTTGAT |