Fig. 2. Thymic cortical T cell deletion induced by a CDR3 cysteine.

TCR-retrogenic mice expressing the 6218 or 6218αC TCR were made as described in Fig. 1, except that all recipients were male B6 mice (n = 4 for 6218; n = 4 for 6218αC). TCR-retrogenic mice were analysed 5 weeks after BM transfer at 99-138 days of age. Thymus sections were stained for GFP (green), with medullary areas identified by staining for cytokeratin-14 (K14, magenta). Dashed lines demarcate cortex (C) from medulla (M); scale bars: 200 μm. Graphs shows the number of GFP⁺ cells per section (top) or per mm² (bottom) in cortical and medullary areas. Each symbol in a graph represents one mouse. P values were determined using unpaired two-tailed t tests. Source data are provided as a Source Data file.