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. 2022 Aug 23;13:4951. doi: 10.1038/s41467-022-32692-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Supernatant IL-2 concentration after coculture of 5KC T cells expressing 6218 or 6218αC and DC2.4 cells with graded concentrations of the peptide denoted in the upper left of each graph. Control cultures included 5KC cells with 50 μM peptide (T only), 5KC and DC2.4 cells without peptide (T + DC), and 5KC cells with plate-bound anti-CD3 (α-CD3). Symbols show the mean, and error bars the range, of 2 wells per condition from one experiment, representative of at least 2 experiments. HillSlope (h) values were determined using nonlinear regression. For curves that did not reach a plateau, the reported EC₅₀ values provide a minimum estimate of the EC₅₀. b For each TCR/peptide combination, graphs show relative tetramer binding (x-axes, plotted as in Fig. 7b) against EC₅₀ (left) or h (right) values, which were determined from a total of at least 4 dilution series per peptide analyzed in at least 2 experiments. Error bars indicate the 95% confidence intervals of EC₅₀ and h; dotted lines connect measurements from the same pMHC. Source data are provided as a Source Data file.