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. 2022 Aug 23;13:4947. doi: 10.1038/s41467-022-32657-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Heatmap of Orc1 ChIP peaks (n = 295) and Mcm2-7 ChIP signal at the corresponding sites. b Examples of Orc1 and Mcm2-7 ChIP data and nucleosome occupancy at six ARS. Red vertical lines represent the location of ACS consensus in these regions. The three ARS on the right contain no consensus with score above 9. c ACS motif score vs. Orc1 ChIP peak strength. The motifs were identified in a 1-kb region near Orc1 ChIP peaks (peak center ±500 bp). All elements with a PWM score >9 are shown here. For the x axis, “1” represents the largest Orc1 ChIP peak, and “295” the weakest. d Fraction of sequences underlying all Orc1 peaks that contain motifs above a certain threshold (varying from 9 to 15). The red arrow represents the recommended cutoff of 11.9. e Same as in d except using a subset of Orc1 peaks that overlap with previously annotated ARS. f Abf1 and Reb1 ChIP peaks analyzed in the same way as in d. The red arrows represent recommended cutoffs for these two factors. g Heatmap of nucleosome occupancy near Orc1 ChIP peaks. The left panel includes 62 peaks containing ACS consensus (PWM > 11.9), with each row aligned at the consensus site. The right panel includes 145 peaks with no consensus above 9, and it was aligned at the center of the ChIP peaks.