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. 2022 Aug 23;8:96. doi: 10.1038/s41523-022-00470-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Western blot analysis of estrogen receptor alpha (ERα) protein expression in breast cancer (BC) cell lines. Cells were cultured for 24 h in the presence of vehicle (−) or fulvestrant 1 µM (+) before lysis. Vehicle = DMSO. ERα protein expression was normalized against β-actin. Increasing levels of hormonal therapy resistance (HTR) are indicated. A potential smaller ERα isoform is indicated in blue. c Comparison between ERα-LBD and ERα-FL (full-length): domains (AF-1, transcription Activation Function-1; DBD, DNA Binding Domain; Hinge; LBD, Ligand Binding Domain; AF-2, transcription Activation Function-2), amino acid (AA) number, molecular weight, 3-D structure prediction (Phyre2). d ERα protein stability assay. ERα-LBD and ERα-FL protein levels were analyzed by western blot in MDA-MB-231 and MCF-7 cells, respectively. Protein samples were collected at different time points (2-24 h), after treatment. Physiological levels of E2 (pM range) were present in all samples, except samples in lane 3 and 4. Vehicle DMSO, CSS charcoal-stripped serum. Source data are provided as a Source Data file.