FIG. 4.

Analysis of xylanase purified from the culture supernatant of T. thermosulfurigenes EM1. (A) Coomassie blue-stained SDS gel; (B) activity staining for xylanases; (C) Western blot prepared with antibodies against the SLH domains of the xylanase. Lanes 1, concentrated culture supernatant; lanes 2, concentrated fraction obtained after affinity chromatography. One to 2 μg of protein was applied in each lane. Five different xylanase forms were observed. Arrows indicate xylanase derivatives containing SLH domains. Numbers on the left indicate migrations of marker proteins (in kilodaltons).