TABLE 1.
Interaction of the xylanases XynA, Xyn-3SLH, and the C-terminally truncated forms Xyn-1SLH and Xyn-ΔSLH with isolated cell wall components of T. thermosulfurigenes EM1
| Xylanase | % of xylanase activity attached to the following cell wall componenta:
|
||||
|---|---|---|---|---|---|
| PG+ | PG− | PGS | SL+ | SL− | |
| XynA | 48 | 18 | 17 | 23 | 9 |
| Xyn-3SLH | 46 | 17 | 11 | 10 | 5 |
| Xyn-1SLH | 7 | 7 | 7 | 8 | 7 |
| Xyn-ΔSLH | 8 | 9 | 8 | 7 | 6 |
a
The xylanase (150 mU) was incubated with 1 mg of the indicated cell wall component in a total volume of 300 μl. The sum of the xylanase activities from the fractions containing bound (p) and unbound protein (s) was defined as 100%.