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. 2022 Aug 3;7(4):1117–1125. doi: 10.1016/j.synbio.2022.07.004

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6 — Improving substrate tolerance of strain to improve (2S)-sakuranetin production.

(A) Effects of overexpressing stress resistance genes on (2S)-sakuranetin production and cell growth when 400 mg/L (2S)-naringenin was added. (B) Effects of overexpressing stress resistance genes on (2S)-sakuranetin production and cell growth when 500 mg/L (2S)-naringenin was added. (C) Effects of overexpressing stress resistance genes on (2S)-sakuranetin production and cell growth when 600 mg/L (2S)-naringenin was added. (D) Spot assay of strain tolerance ability to (2S)-naringenin. (E) The growth status and production of (2S)-sakuranetin when rpsQ^His31Pro and secB were co-expressed with rpoS. ****P < 0.0001.