Table 1.
Strains used in the present study.
| Strains | Description | Source |
|---|---|---|
| E. coli JM109 |
recA1, endA1, gyrA96, thi-1, hsdR17 (rk-mk+), e14-(mcrA-) supE44, relA1, Δ (lac-proAB)/F’[traD36, proAB+, lacIq, lacZΔM15] |
Sangon Biotech (Shanghai) |
| E. coli BL21 (DE3) | F-ompT hsdSB (rB-mB-) gal dcmrne131(DE3) | Sangon Biotech (Shanghai) |
| 7-FOMT-1 | E. coli BL21(DE3) carrying pET-28a (+)-OsNOMT | This study |
| 7-FOMT-2 | E. coli BL21(DE3) carrying pET-28a (+)-SaOMT2 | This study |
| 7-FOMT-3 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3 | This study |
| NS01 | E. coli BL21(DE3) carrying pCDFDuet-metA-cysE, pET-28a (+)-PfOMT3 | This study |
| NS02 | E. coli BL21(DE3) carrying pCDFDuet-metA-cysE-metK, pET-28a (+)-PfOMT3 | This study |
| NS03 | E. coli BL21(DE3) carrying pCDFDuet-metA-cysE-ydaO, pET-28a (+)-PfOMT3 | This study |
| NS04 | E. coli BL21(DE3) carrying pCDFDuet-metA-cysE-ydaO-metK, pET-28a (+)-PfOMT3 | This study |
| NS07 | E. coli BL21(DE3) carrying pACYCDuet-SNZ3, pET-28a (+)-PfOMT3 | This study |
| NS08 | E. coli BL21(DE3) carrying pACYCDuet-RPS18B, pET-28a (+)-PfOMT3 | This study |
| NS09 | E. coli BL21(DE3) carrying pACYCDuet-RFC4, pET-28a (+)-PfOMT3 | This study |
| NS10 | E. coli BL21(DE3) carrying pACYCDuet-SNZ3-RPS18B, pET-28a (+)-PfOMT3 | This study |
| NS11 | E. coli BL21(DE3) carrying pACYCDuet-SNZ3-RPS18B-RFC4, pET-28a (+)-PfOMT3 | This study |
| NS14 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-POS5 | This study |
| NS15 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-POS5, pCDFDuet-metA-cysE-ydaO | This study |
| NS16 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-POS5, pACYCDuet-SNZ3-RPS18B-RFC4 | This study |
| NS17 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3, pCDFDuet-metA-cysE-ydaO, pACYCDuet-SNZ3-RPS18B-RFC4 | This study |
| NS18 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-POS5, pCDFDuet-metA-cysE-ydaO, pACYCDuet-SNZ3-RPS18B-RFC4 | This study |
| NS19 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-rpsL | This study |
| NS20 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-rpsQ | This study |
| NS21 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-rpsQHis31Pro | This study |
| NS22 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-crp | This study |
| NS23 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-rpoS | This study |
| NS24 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-secB | This study |
| NS25 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-nfuA | This study |
| NS26 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-yajL | This study |
| NS27 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-ycdy | This study |
| NS28 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-proQ | This study |
| NS29 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-nusB | This study |
| NS30 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-acrR | This study |
| NS31 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-asr | This study |
| NS32 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-rpsQHis31Pro-rpoS | This study |
| NS33 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-secB-rpoS | This study |
| NS34 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-rpoS, pCDFDuet-metA-cysE-ydaO, pACYCDuet-SNZ3-RPS18B-RFC4 | This study |
| NS35 | E. coli BL21(DE3) carrying pET-28a (+)-PfOMT3-secB-rpoS, pCDFDuet-metA-cysE-ydaO, pACYCDuet-SNZ3-RPS18B-RFC4 | This study |