Fig. 4.

Glycogen storage was reduced in female Gulo^−/− pups after VC deprivation. Female Gulo^−/− pups (5 pups per group) were supplemented with 3.3 g/L VC or devoid of VC in the drinking water for 3, 4, or 5 weeks at 22 days old. The following experiments required the collection of livers. (A-C) PAS staining in paraffin-embedded liver sections and representative images. (D) Hepatic glycogen levels as measured by biochemistry. Mean SD, n = 5, one-way ANOVA and Dunnett's multiple comparison tests; *p < 0.05, **p < 0.01 versus VC+. Square frames define the magnified regions.