Fig. 2.
Derivation and long-term culture of embryo derived hNPPCs. (A) Schematic diagram showing the isolation of human embryo derived hNPPCs. (B) The total embryo dissected and their gestation ages as well as the derivation efficiency. (C) Number of hNPPCs after 30 passages (starting from 5.4 × 105 cells). (D) Bright field 10X images of cultured mNPPCs from embryos at different passages. Scale bar = 100 μm. Bright field 200X images of cultured mNPPCs from embryos at different passages. Scale bar = 2000 μm (E) FACS analysis of hNPPCs' expression of Tie2. (F) FACS analysis of indicated surface markers on hNPPCs (red line) compared with hiPSCs (blue line). The grey column indicated the IgG for each antibody. (G) Western blot analysis of indicated proteins between hiPSCs and hNPPCs. (H) Immunofluorescence results (20X) of hNPPCs with Aggrecan, Collagen II, Collagen I and SOX9 expression. Scale bar = 100 μm. (I) The quantitative result of Fig. 2G. **p < 0.01, *p < 0.05 vs. hiPSCs. (Student's t-test).