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. 2022 Aug 10;10:937595. doi: 10.3389/fbioe.2022.937595

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Copyright © 2022 Xu, Jiang, Gu, Li, Xu, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fabrication and characterization of MPCS. Schematic diagram of (A) the fabrication process for perforated PDMS film, (B) PEG coating, and (C) cell seeding on the MPCS. (D) Photograph of MPCS. (E) Bright field images of MPCS with circular patterns of 500 μm, 200 μm, and 100 μm in diameter from left to right, respectively. (F) Scatter plot (mean ± SD, n = 20), (G) Relative error (n = 20), and (H) CVs of actual diameters of the perforated PDMS film (n = 6). Data are the mean ± SD and analyzed by One-way analysis of variance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.