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. 2022 Aug 10;10:937595. doi: 10.3389/fbioe.2022.937595

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Copyright © 2022 Xu, Jiang, Gu, Li, Xu, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of hiPSC-derived mpHLOs. (A) Culture timeline diagram of mpHLOs. (B)Time-lapse images of generation of mpHLOs. mpHLOs are circular patterns with a diameter of 500 μm. (C) Schematic diagram of mpHLOs. (D) Bright-field images of mpHLOs in 24-well plate. (E) HLOs in Matrigel dome. (F) Normalized area of HLOs (n = 20). (G) CVs of HLOs (n = 6). Data are the mean ± SD and analyzed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.