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. 2022 Aug 10;10:937595. doi: 10.3389/fbioe.2022.937595

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Copyright © 2022 Xu, Jiang, Gu, Li, Xu, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry analysis of mpHLOs. (A) Immunofluorescence staining of hiPSCs and FSCs for pluripotency markers NANOG and OCT4, posterior foregut marker CDX2, epithelial marker EpCAM. Immunofluorescence staining of mpHLOs for (B) hepatic maker HNF4α and mesenchymal marker VIM, (C) hepatic marker ALB, (D) epithelial marker EpCAM and hepatoblasts marker AFP, and (E) cholangiocyte marker CK19. (F–K) Semi-quantification analysis of immunofluorescence staining. Data are presented as the mean ± SD (n = 3) and analyzed by One-way analysis of variance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.