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. 2022 Aug 10;10:937595. doi: 10.3389/fbioe.2022.937595

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Copyright © 2022 Xu, Jiang, Gu, Li, Xu, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional characterization of mpHLOs. (A) Glycogen storage of mpHLOs. (B–C) Albumin and urea secretion during two-week differentiation. (D) Lipid accumulation of mpHLOs. (E) Heat map indicates differential gene expression analysis of liver specific function genes. (F–H) Relative mRNA expression of CYP450 enzymes (CYP3A4, CYP2E1, and CYP1A2) after 48 h treatment with 0 and 20 mM APAP. Data are shown as the fold changes in expression relative to the levels without treatment. Data are the mean ± SD (n = 3) and analyzed by Student’s t test or One-way analysis of variance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.