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. 2022 Aug 10;13:973986. doi: 10.3389/fimmu.2022.973986

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Copyright © 2022 Baumgaertner, Schmidt, Costa-Nunes, Bordry, Guillaume, Luescher, Speiser, Rufer and Hebeisen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hierarchical analysis of TCR-pHLA K_D affinity versus peptide:HLA K_i affinity. (A) Curves obtained from parallel cytotoxic killing assays of primary CD8 T cells expressing five NY-ESO-1_157-165-specific TCRs of incremental affinities (very low affinity K_D of V49I < 100μM, wild type K_D = 21.4 μM, optimized DMβ = 1.91 μM and TMβ = 0.91 μM and very high affinity TCR wtc51 = 0.015 μM) against 8 NY-ESO-1_157-165 peptide variants of increasing (n = 4 to 8). (B) Functional avidity values (EC₅₀) obtained from the parallel killing assays of the NY-ESO-1_157-165-specific T cells plotted against the K_D affinity of the TCR for the pHLA. (C) Correlation analysis between the functional avidity values (EC₅₀) of the NY-ESO-1_157-165-specific T cells and the K_i affinity of the respective target peptides. R square values are displayed.