Table 1.
Sequences, algorithm-based and biophysical values of HLA-A*0201-restricted Melan-A/MART126-35 P2/P10 peptide variants and Flu Matrix58-66 control.
| Melan-A/MART-126-35 | |||||||
|---|---|---|---|---|---|---|---|
| peptide variant | Sequence (decamer) | IEDB pred. | NetMHC 4.0 | PRIME 2.0 | refolding assay | Peptide IC50 | Ki affinity* |
| n° | P2 P10 | % rank | μM | % rank | O.D. | μM | μM |
| 1 (native) | E-A-A-G-I-G-I-L-T-V | 13.5 | 5.32 | 0.74 | 0.32 | 22.50 | 4.67 |
| 2 | E-A-A-G-I-G-I-L-T-L | 22 | 9.30 | 0.92 | 0.10 | 117.00 | 24.47 |
| 3 | E-A-A-G-I-G-I-L-T-A | 24 | 13.78 | 1.68 | 0.10 | 133.00 | 27.68 |
| 4 | E-A-A-G-I-G-I-L-T-I | 30 | 10.21 | 1.44 | 0.11 | 30.70 | 6.39 |
| 5 | E-A-A-G-I-G-I-L-T-M | 30 | 13.14 | 2.25 | 0.10 | 284.00 | 59.14 |
| 6 (analog) | E-L-A-G-I-G-I-L-T-V | 1.8 | 0.25 | 0.11 | 0.54 | 2.48 | 0.52 |
| 7 | E-M-A-G-I-G-I-L-T-V | 4.1 | 0.23 | 0.26 | 0.60 | 3.60 | 0.75 |
| 8 | E-I-A-G-I-G-I-L-T-V | 6.65 | 1.93 | 0.28 | 0.54 | 3.35 | 0.70 |
| 9 | E-V-A-G-I-G-I-L-T-V | 6.7 | 3.15 | 0.36 | 0.63 | 4.21 | 0.88 |
| 10 | E-L-A-G-I-G-I-L-T-L | 1.9 | 0.82 | 0.14 | 0.44 | 4.17 | 0.87 |
| 11 | E-L-A-G-I-G-I-L-T-A | 4.95 | 1.60 | 0.32 | 0.43 | 5.61 | 1.17 |
| 12 | E-L-A-G-I-G-I-L-T-I | 4.5 | 0.91 | 0.26 | 0.71 | 2.93 | 0.61 |
| 13 | E-L-A-G-I-G-I-L-T-M | 7.15 | 2.14 | 0.47 | 0.10 | 36.80 | 7.66 |
| 14 | E-M-A-G-I-G-I-L-T-L | 4.45 | 0.86 | 0.34 | 0.56 | 4.38 | 1.01 |
| 15 | E-M-A-G-I-G-I-L-T-A | 7.3 | 1.79 | 0.64 | 0.55 | 3.62 | 0.75 |
| 16 | E-M-A-G-I-G-I-L-T-I | 7.85 | 0.98 | 0.54 | 0.71 | 1.79 | 0.37 |
| 17 | E-M-A-G-I-G-I-L-T-M | 8.9 | 2.40 | 0.94 | 0.12 | 37.50 | 7.81 |
| FluMA58-66 | G-I-L-G-F-V-F-T-L | 0.80 | 0.016 | 0.006 | 1.00 | 1.02 | 0.21 |
* Blue peptide KD = 52.6 nM.