FIGURE 1.

Schematic of EV miRNA isolation based on Fe₃O₄@TiO₂. (A) Principle for EV isolation based on Fe₃O₄@TiO₂. After capture, miRNAs were released from the EVs by the heat-lysis method. (B) Process of manual EV miRNA isolation. The sample was incubated with Fe₃O₄@TiO_2, and the beads were separated with magnets. Then, the supernatant was discarded after cleaning. Elution buffer was added to the beads to extract miRNAs. (C) Process of automated EV miRNA isolation. Binding buffer (pink), cleaning buffer (blue), and elution buffer (green).