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. 2022 Aug 10;10:948757. doi: 10.3389/fbioe.2022.948757

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Copyright © 2022 Di, Fan, Gu, Huang, Khan, Liu, Shen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Automated EV isolation and miRNA extraction. (A) Internal structure of the automated instrument. (B) Optimization results for elution temperature. (C) Optimization results for mixing speed. (D) Copy number of miRNAs from different EV miRNA extraction methods. (E) CT values from EV miRNA extraction in different matrixes. (F) Recovery rate for magnetic beads by the manual method and automatic method (*p < 0.05, **p < 0.01, and ***p < 0.001, ns, non-significant).