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Metabolic pathway involved in PA production from LA in P. putida. The yciA (encoding thioesterase from H. influenzae) and ygfH (encoding propionyl-CoA: succinate CoA transferase from E. coli) were overexpressed, and the prpC and prpE genes encoding methylcitrate synthase and propionyl-CoA synthase, respectively, were deleted. MC cycle, methylcitrate cycle; TCA cycle, tricarboxylic acid cycle; lvaABCDE, lva operon of P. putida involved in LA degradation.
