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. 2022 Aug 10;10:939248. doi: 10.3389/fbioe.2022.939248

TABLE 1.

Strains and plasmids used in this invention.

Strain and plasmids Description Source
E. coli DH10B Cloning host Lab stock
P. putida EM42 Derivative strain from wild type P. putida KT2440 Martínez-García et al. (2014)
EM42:ΔC EM42 with deleted prpC This study
EM42:ΔE EM42 with deleted prpE This study
EM42:ΔCE EM42 with deleted prpC and prpE This study
EM42:ΔCE:yciA EM42:ΔprpCprpE harboring pPROBE_ LvaR/P lvaA _ yciA This study
EM42:ΔCE:ygfH EM42:ΔprpCprpE harboring pPROBE_LvaR/P lvaA _ygfH This study
EM42:ΔCE:yciA:ygfH EM42:ΔprpCprpE harboring pPROBE_LvaR/P lvaA _yciA_ygfH This study
pPROBE_ LvaR/P lvaA _egfp + pBBR1-ori, carrying egfp + under the control of LvaR/P lvaA (levulinic acid inducible promoter) from P. putida KT2440, KmR Sathesh-Prabu et al. (2021)
pPROBE_ LvaR/P lvaA _ yciA pPROBE_LvaR/P lvaA with Δegfp +:yciA from Haemophilus influenzae DSM 11121 This study
pPROBE_ LvaR/P lvaA _ygfH pPROBE_LvaR/P lvaA with Δegfp +:ygfH from E. coli DH10B This study
pPROBE_ LvaR/P lvaA _ yciA_ygfH pPROBE_LvaR/P lvaA with Δ egfp + egfp +:yciA:ygfH This study
pQSAK ColE1-ori, sacB, KmR and AmpR Zhou et al. (2014)
pQSAK-prpC used to delete prpC in EM42 This study
pQSAK-prpE used to delete prpE in EM42 This study