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. 2022 Aug 10;16:963813. doi: 10.3389/fnins.2022.963813

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Copyright © 2022 Hu, Li, Huang, Li, Xu, Xu, Chen, Wang, Deng, Zhu, Feng and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STAMBP expression during human cortical development. (A) STAMBP expression in the human cortex, hippocampus, and cerebellum. Data were downloaded from Allen Brain Atlas (available from: http://www.brain-map.org; Ensemble ID: ENSG00000124356). pcw, post-conceptional weeks; mos, months; yrs, years. (B) Schematic representation of the culture system described in detail in Section “Patient and methods.” Examples of each stage are shown. Scale bars, 200 μm. (C) Immunofluorescent staining of hCOs of day 21 for neural stem cell markers Left column: SOX2, PAX6, and DAPI; middle column: SOX2, DCX, and DAPI; right column: SOX2, NeuN, and DAPI. Scale bars, 100 μm. (D) STAMBP mRNA expression in the different differentiated stages of hCOs. Mean ± SEM from three independent experiments.