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. 2022 Aug 10;13:960279. doi: 10.3389/fendo.2022.960279

Figure 3.

Figure 3

Dexamethasone stimulates expression of lipolytic enzyme Atgl in WAT, which is prevented by corticosterone add-on treatment. Mice received a high-fat diet mixed with dexamethasone (DEX) or vehicle (VEH) and were subcutaneously implanted with low-dose (5 mg) corticosterone (CORT) or vehicle pellets for 3.5 weeks. DEX decreased tissue weight and adipocyte size, as determined by H&E staining, in (A–C) subcutaneous white adipose tissue (sWAT) and in (D–F) gonadal white adipose tissue (gWAT). (G) In sWAT, DEX increased the expression of lipolytic enzyme Atgl and browning markers Prdm16, Pgc1a and aP2. DEX + CORT prevented the DEX-induced upregulation of Atgl expression. (H) In gWAT, DEX did not affect Atgl, Hsl, or Cd36/FAT expression, but decreased Lpl expression. Statistical significance was calculated using 2-way ANOVA analysis. Depicted below the graphs are the P-values of the ANOVA tests for either CORT (C) or DEX (D) treatment or the interaction between CORT and DEX (I). *P < 0.05, ** P < 0.01, ***P < 0.001. 'ns' means 'not significant'.