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. 2022 Aug 10;13:905703. doi: 10.3389/fendo.2022.905703

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Copyright © 2022 Jiang, Xu, Yao, Zhang, Li, Zhang, Xie, Xing, Zhang, Zhou, Liao and Dong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Podocyte apoptosis was detected by TUNEL staining with different glucose concentrations. (A) Podocyte apoptosis rate increased significantly in response to high glucose (25 mM). White arrows point to TUNEL-positive podocytes (apoptotic podocytes); magnification: ×200; scale bar = 25 μm. (B) Absolute count of TUNEL-positive podocytes. Data were expressed as the mean numbers of apoptotic podocytes from 20 randomly selected fields and were from at least three independent experiments. Data are presented as mean ± SEM; *P < 0.05; ns, not significant.