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. 2022 Aug 10;13:926650. doi: 10.3389/fimmu.2022.926650

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Copyright © 2022 Wenzlau, DiLisio, Barbour, Dang, Hohenstein, Nakayama, Delong, Baker and Haskins

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B-chain HIP crosslinking library scheme and IFN-γ ELISAs for T cell clone BDC-6.3 and PD12-4.4. (A) In the Left Library, 11 individual B-chain peptides form peptide bonds with two pools of right secretory granule peptides (pools R1-14, R15-28). The Right Library is comprised of 28 individual right peptides that form peptide bonds with the pool of 11 B-chain left peptides. (B, C) Heat maps for IFN-γ ELISA screening of T cell clones BDC-6.3 and PD12-4.4 with B chain Right and Left HIP libraries. Data is shown as absorption @ 415 nm minus background for a single screen for each T cell clone.