Skip to main content
. 2022 Aug 10;13:926650. doi: 10.3389/fimmu.2022.926650

Figure 2.

Figure 2

BDC-6.3 IFN-γ response to candidate B-chain HIP antigens. (A) Synthetic peptides representing the highest scoring peptides within the Left and Right peptide libraries. Blue = B:9-23 derived peptides, red italics = natural cleavage product peptides from other ß-cell proteins. The PreProSAAS peptide is 14 amino acids spanning a dibasic proteolytic processing site and was included as a negative control. (B) IFN-γ responses of T cell clone BDC-6.3 to 10 µM concentrations of B:9-23 or synthetic B-chain HIP candidates. (C) IFN-γ responses of T cell clone PD12-4.4 to 10 µM concentrations of B:9-23 or synthetic B-chain HIP candidates. Compiled data is shown for the average of 3-8 experiments (BDC-6.3) or 3-7 experiments (PD12-4.4), with statistical analysis using a one-way ANOVA with Dunnett’s multiple comparison test *P<0.05, ****P<0.0001 where the box defines the interquartile range, and the whiskers indicate minimum and maximum values.