Fig. 2.

BiTEs activated untransduced T cells in HEK293T cells. (A) Schematic diagram of three CARs used in this study. (B) Transduction efficiency of GPC3 CAR and GPC3-BiTE CAR on HEK293T cells was detected by flow cytometry. (C) Expression of BiTEs in HEK293T cells were detected by ELISA. (D) Expression analysis of CD69 was detected by flow cytometry. (E) Expression of IFN-γ was detected by flow cytometry using CBA kit. MFI: Mean Fluorescence Intensity. Statistical significance was assessed by t-test (C) and one-way ANOVA (D and E). Not significant ns, P > 0.05, *P ≤ 0.05, ***P ≤ 0.001.