Table 1:
Summary of recently reported mtDNA enrichment methods for sequencing.
| Enrichment Method |
Input DNA |
mtDNA Enrichment (cell line) |
Media n read length |
Steps in Enrichment |
Sequencin g Method |
DNA Amplificati on |
|---|---|---|---|---|---|---|
| nCATs-mtDNA | 3 ug | 74% | 16456 bp | 3 | Oxford Nanopore | No |
| Exo/Endonuclea se and Column Prep(Lujan et al. 2020) | 200 ng | 60% | 150 bp | 3 | Illumina NextSeq | Yes |
| Exonuclease and Rolling Circle Amplification(Y ao et al. 2019) | 25 ng | 95% | 200 bp | 4 | Ion PGM | Yes |
| Long Range PCR(Zhang, Cui, and Wong 2012) | 100 ng | Not provided | 100 bp | 3 | Illumina HiSeq | Yes |