Figure 3.

Effect of neutrophil depletion on heart function and vascular morphology 10 weeks after therapy. (A) In mice treated with control phosphate-buffered saline(PBS), doxorubicin(DOX), DOX plus control IgG, or DOX plus anti-Ly6G, ejection fraction(EF) and fractional shortening(FS) were quantified by echocardiography and followed for 10 weeks after therapy; (B) Left ventricular internal dimension in systole (LVID(s)) was also quantified 10 weeks after therapy; (C) Representative images and numbers of NG2⁺ and CD31⁺ vessels in the hearts of mice treated with control phosphate-buffered saline (PBS), doxorubicin (DOX), DOX plus control IgG, or DOX plus anti-Ly6G 10 weeks after therapy; (D) Collagen deposition in the hearts was determined using Masson trichrome staining 10 weeks after therapy completion. Data are presented as mean ± SEM, n = 5 each, *p < 0.05, one-way ANOVA analysis with Tukey comparison was used to compare the groups.