Figure 1.

Bantam promotes nighttime sleep via the γ5β′2a/β′2mp/β′2mp_bilateral MBONs

(A) Adult-specific knockdown of ban. Sleep data for nSyb>ban-SP, tubulin-GAL80^ts (N = 27) and scramble controls (N = 31) raised at 17°C and tested at 29°C (mean ± SEM). ∗ represents p ≤ 0.0001, Mann-Whitney-Wilcoxon test or unpaired t-test.

(B) Anti-GFP staining (green) for the central brain of a representative ban>CD8::GFP fly (63X). Anti-BRP (nc82) was used to stain neuropil (red). Scale bar measures 25μm.

(C) Anti-GFP (green) and anti-BRP (red) staining for the brain of a representative MB011B>UAS-CD8::GFP fly (63X). Scale bar measures 25μm.

(D) Sleep data for MB011B>ban-SP (N = 17) and scramble controls (N = 24) shown as mean± SEM ∗ represents p ≤ 0.0005, unpaired t-test.

(E) Sleep latency for same animals (minutes to fall asleep after lights out) shown as mean± SEM ∗ represents p ≤ 0.05, unpaired t-test.

(F) Beam breaks per active minute (general locomotor activity) for same animals (mean± SEM).