Figure 3.

Bantam inhibits neural activity in the wake-promoting γ5β′2a/β′2mp/β′2mp_bilateral MBONs

(A) Schematic diagram of paradigm for antennal lobe (AL) microelectrode stimulation and γ5β′2a/β′2mp/β′2mp_bilateral MBON GCaMP6f recording. Dashed line represents mushroom body.

(B) GCaMP6f images showing representative dendritic Ca²⁺ responses of GMR14C08>scr-SP+GCaMP6f during baseline and after antennal lobe stimulation. Experiment was carried out at ZT12:40. 0.12mA stimulation was delivered. Scale bar measures 50μm.

(C) Average ΔF/F time course for Ca²⁺ responses of GMR14C08>scr-SP+GCaMP6f (gray) and GMR14C08>ban-SP+GCaMP6f (red) dendrites to antennal lobe stimulation (orange arrow) in the ZT12-14 time window (mean± SEM).

(D) ΔF/F for Ca²⁺ responses of GMR14C08>scr-SP+GCaMP6f (gray) and GMR14C08>ban-SP+GCaMP6f (red) dendrites to antennal lobe stimulation (0.12mA) in the ZT0-2, ZT6-8 and ZT12-14 time windows (mean± SEM). ∗ represents p ≤ 0.05, two-way ANOVA and Sidak post hoc comparison. n = 8 for all groups.

(E) Sleep data for MB011B>dTrpA1 (n = 31), MB011B control (n = 27) and UAS-dTrpA1 control (n = 30) tested for sleep behavior at 29°C (mean± SEM). ∗ represents p ≤ 0.01, Kruskal-Wallis test with Dunn’s multiple comparison test.

(F) Sleep data for GMR14C08>ban-SP+shi^ts (n = 30) and GMR14C08>scr-SP+shi^ts (n = 31) tested at 18°C and 29°C (mean± SEM). ∗ represents p ≤ 0.01, Mann-Whitney-Wilcoxon test.