REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD3	BioXCell	Clone:145–2C11; Cat#BE0001–1; RRID:AB_1107634
Anti-mouse CD28	BioXCell	Clone:37.51; Cat#BE0015–1; RRID:AB_1107624
Anti-mouse FoxP3-PE	eBioscience	Clone:FJK-16s; Cat#12-5773-82
Anti-mouse CD4-PE/Cy7	Biolegend	Clone:GK5.1; Cat#100422; RRID:AB_312707
Anti-mouse CD4-APC	Biolegend	Clone:GK5.1; Cat#100412; RRID:AB_312697
Anti-mouse CD44-APC	Biolegend	Clone; IM7; Cat#103012; RRID:AB_312963
Anti-mouse IFN-γ-PE	Biolegend	Clone: XMG1.2; Cat#505808; RRID:AB_315402
Anti-mouse TNF-α-PE/Cy7	Biolegend	Clone: MP6-XT22; Cat#506306; RRID:AB_315427
Anti-mouse CD69-PE	Biolegend	Clone: H1.F3; Cat#104508; RRID:AB_313111
Anti-mouse TGF-β	Biolegend	Cat#580702
Anti-mouse CD8-BV510	Biolegend	Clone: 53–6.7; Cat#100752; RRID:AB_2563057
Biotinylated anti-mouse CD19	Biolegend	Cat#101504; RRID:AB_312823
Biotinylated anti-mouse CD11b	Biolegend	Cat#101204; RRID:AB_312787
Biotinylated anti-mouse CD11c	Biolegend	Cat# 117304; RRID:AB_313773
Biotinylated anti-mouse NK1.1	Biolegend	Cat# 108704; RRID:AB_313391
Biotinylated anti-mouse CD25	Biolegend	Cat# 102004; RRID:AB_312853
Chemicals, peptides, and recombinant proteins
Streptavidin magnetic beads	BD Biosciences	Cat#557812
IL-2	Frederick National Laboratory for Cancer research	Tecin(teceleukin); Ro 23-6019
Retinoic acid	Sigma	Cat# R2625-50MG
RANKL	R&D Systems	Cat# 462-TEC-010
M-CSF		Cat# 576,404
Collagenase-P	Roche Diagnostic Corporation	Cat#11249002001
IMDM media	Hyclone	Cat#SH30228.01
Alpha-MEM	Gibco	Cat#12571-048
Fetal Calf Serum	AtlantaBiologicals	Cat#S11150
Fetal Bovine Serum	Gibco	Cat#26140-079
L-glutamine	Sigma	Catalog #G8540-100G
Penicillin/Streptomycin	AtlantaBiologicals	Catalog #B21210
Beta-mercaptoethanol	Thermo Scientific	Catalog # 35602
Ascorbic acid 2-phosphate	Wako Pure Chemical Industry	Cat# O19-12063
β-glycerophosphate	Sigma	Cat# 157241
PMA	Sigma	Cat# P8039-1MG
Ionomycin	Sigma	Cat# I0634-1MG
GolgiPlug™	BD Biosciences	Cat# 555029
Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE)	Molecular Probes; Invitrogen	Cat# C1157
BD Cytofix/Cytoperm Kit	BD Biosciences	Cat# 554714
Trizol	Life Technologies	Cat# 15596026
iScript cDNA Synthesis Kit	BioRad	Cat#1725035
TaqMan Gene Expression Assay NFATc1	Thermo Fisher	Cat#4453320; Mm00479445_m1
TaqMan Gene Expression Assay CTSK	Thermo Fisher	Cat#4453320; Mm00484039_m1
TaqMan Gene Expression Assay TRAP	Thermo Fisher	Cat#4453320; Mm00475698_m1
TaqMan Gene Expression Assay Runx-2	Thermo Fisher	Cat#4453320; Mm00501584_m1
TaqMan Gene Expression Assay ALP	Thermo Fisher	Cat#4453320; Mm00475834_m1
TaqMan Gene Expression Assay Osteocalcin	Thermo Fisher	Cat#4453320; Mm03413826_mH
TaqMan Gene Expression Assay TGF-β receptor I	Thermo Fisher	Cat#4453320; Mm00436964_m1
TaqMan Gene Expression Assay TGF-β receptor II	Thermo Fisher	Cat#4453320; Mm03024091_m1
TaqMan Gene Expression Assay β-Actin	Thermo Fisher	Cat#4453320; Mm02619580_g1
Neomycin	Sigma	Cat #N1876-100G
Cetylpyridinium chloride	Sigma	Cat# C0732-100G
Sodium phosphate	Sigma	Cat# 342483
Sodium hypochlorite	Sigma	Catalog # 7681-52-9
Critical commercial assays
Mouse IFN-γ ELISA kit	Biolegend	Cat#430801
Mouse TNF-α ELISA kit	Biolegend	Cat#430901
Alizarin Red Staining Kit	EMD Millipore Corporation	Cat#3070121
Leukocyte acid phosphatase kit	Sigma Aldrich	Catalog #387A-1KIT
Bone Resorption Kit	CosmoBio	Cat# CSR-BRA-48X2KIT
Experimental models: Organisms/strains
oim/oim (B6C3Fe-a/a-Col1a2oim/J)	Jackson Laboratory	Cat# 001815
FoxP3.GFP mice (C.Cg-Foxp3tm2Tch/J)	Jackson Laboratory	Cat#006769
C57Bl/6J mice	Jackson Laboratory	Cat#000664
Software and algorithms
FlowJo Software	BD	https://www.flowjo.com/solutions/flowjo/downloads/
CT Bone Visualization and Analysis	Siemens Medical Solution Medical Solutions	https://sharedimaging.com/
Analyze 12.0 software for Micro-CT	AnalyzeDirect	https://analyzedirect.com/
Electro force system	EnduraTec	ELF3200
Bioquant OSTEO II software platform	Bioquant Image Analysis Corporation	https://bioquant.com/
GraphPad Prism 6.0 software	GraphPad software Inc.	https://www.graphpad.com/scientific-software/prism/
Other
Irradiated mice diet	Tekland Global Diets
Isofluorane	Piramal	Cat# NDC 66794-017-25