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. Author manuscript; available in PMC: 2022 Aug 24.
Published in final edited form as: Methods Mol Biol. 2015;1335:205–221. doi: 10.1007/978-1-4939-2914-6_14

Fig. 4.

Fig. 4.

CNO treatment of mice expressing Dq in AgRP neurons (AgRP-Dq mice) triggers a dramatic increase in food intake. AgRP-Dq mice received a single i.p. injection of either saline or CNO (1 mg/kg) in the morning (between 9 and 10.30 a.m.). AgRP-Dq mice display a significant increase in food intake during the first few hours after CNO treatment (A) and after day 1 (B). This CNO-dependent orexigenic effect is no longer observed on day 2 after CNO injection (B). The data shown are from a representative experiment (K. Nakajima et al., unpublished results). Data represent means ± SEM (n=8 per group; 10–11-week-old male mice). ***p<0.001, as compared to the corresponding control group.