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. 2022 Jul 8;24(3):563. doi: 10.3892/etm.2022.11500

Figure 8.

Figure 8

LTBP2 inhibits the proliferation, migration and invasion of PCa cells in vitro. (A) RT-qPCR assay showed the mRNA expression levels of LTBP2 in prostate cells (RWPE-1) and PCa cell lines (LNcap and DU145). (B) Western blot assay showed the protein expression levels of LTBP2 in prostate cells (REPW-1) and PCa cell lines (LNcap and DU145). (C) Relative expression of LTBP2 by RT-qPCR in PCa cells transfected with OE-LTBP2 and NC. (D) Relative expression of LTBP2 determined by western blotting in PCa cells transfected with OE-LTBP2 and NC. (E) Transwell assays showed the migration and invasive abilities of PCa cells transfected with OE-LTBP2 and NC. (F) CCK-8 assays showed the proliferation capacity of PCa cells transfected with OE-LTBP2 and NC. (G) Western blot assay displayed the protein levels changes in PCa cells transfected with OE-LTBP2 and NC. All experiments were repeated three times. *P<0.05, **P<0.01. LTBP2, latent transforming growth factor β-binding protein 2; PCa, prostate cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; OE, overexpressed; NC, negative control; CCK-8, Cell Counting Kit-8.