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. 2022 Aug 23;14:105. doi: 10.1186/s13148-022-01327-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Integrated and validated analysis for gene expression profiling and DNA methylation. A Four-quadrant diagram shows DMRs and the expression of corresponding DEGs. Genes with yellow color are hypermethylated downregulated DEGs, and genes with green color are hypomethylated upregulated DEGs. B Dot plot of the relative expression level of screened six genes. *P < 0.05, **P < 0.01. The results of pyrosequencing of IGF2BP1 (C) and FLT1 (D)