Fig. 8.

Cytokines profile following vaccination. Immunized mice were sacrificed 14 days after the last vaccination. Splenocytes were prepared and stimulated with the FNOM and S proteins for 72 h. The cytokines profile of the TNF-α, IFN-γ, IL-10, IL-17A, IL-17F, IL-6 and IL-22 were assessed. Compared to control and S protein groups, in subjects receiving the FNOM and S + FNOM vaccine candidates, the levels of TNF-α, IFN-γ, IL-10 and IL-17A cytokines were increased after the spleen cells stimulation. This increase was significant for TNF-α and IFN-γ cytokines (p < 0.001).