FIG 5.

Cleaved HDAC2 loses the ability to induce ISGs. LLC-PK1 cells (A) and HEK-293T cells (B) were transfected with expression plasmids for WT HDAC2, HDAC2-Δ1-261, HDAC2-Δ262-488, or empty vector along with ISRE-Luc plasmid and pRL-TK plasmid. After 24 h, cells were treated with 1,000 U/mL of IFN-α for 12 h, followed by dual-luciferase assays. (C–E) LLC-PK1 cells were treated as described above. At 12 h IFN-α stimulation, the cells were collected for qRT-PCR for the purpose of detecting mRNA expression of ISG15 (C), ISG54 (D), and ISG56 (E). (F–H) LLC-PK1 cells were mock-treated or pretreated with CAY (75 μM) for 2 h, followed by PDCoV infection (MOI 0.5). At 24 h after infection, cells were collected for qRT-PCR for the purpose of detecting mRNA expression of ISG15 (F), ISG54 (G), and ISG56 (H). All experiments were performed in triplicate, and the data are presented as means ± SD of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.