FIG 3.
1918 influenza virus infection of Mauritian cynomolgus macaques by multiple doses and routes of exposure. Group 1 animals (n = 4) were infected with 5 × 104 PFU via intrabronchial inoculation, group 2 animals (n = 4) were infected with 5 × 105 PFU via intrabronchial inoculation, and group 3 animals (n = 4) were infected with 7 × 106 PFU via intranasal, ocular, oral, and intratracheal inoculation. Weight loss (A), heart rate (B), respiratory rate (C), oxygen saturation (SPO2) (D), rectal temperature (E), and clinical score (F) were monitored for the duration of the study for all animals. Data were compared by two-way ANOVA with Tukey’s multiple-comparison test between the groups. Symbols for group 1 versus group 2 (#) and group 1 versus group 3 (*) represent P values of <0.05.