| Antibodies |
|
| CD144 (VE-Cadherin) MicroBeads |
MiltenyiBiotec |
130-097-857 |
| anti-phospho-NF-kB p65 antibody |
Cell Signaling |
#3033; AB_2341216 |
| anti-NF-κB p65 antibody |
Cell Signaling |
#8242; AB_10859369 |
| anti-IKB alpha antibody |
Abcam |
ab32518; AB_733068 |
| anti-phospho-IκBα antibody |
Cell Signaling |
#9246; RRID: AB_2267145 |
| anti-TLR4 antibody |
Abcam |
ab89455; RRID: AB_2043061 |
| anti-MyD88 antibody |
Cell Signaling |
#3699; RRID: AB_2282236 |
| anti-p38 MAPK antibody |
Cell Signaling |
#8690; RRID: AB_10999090 |
| anti-phospho-p38 MAPK antibody |
Cell Signaling |
#9211; RRID: AB_331641 |
| anti-F4/80 antibody |
Abcam |
ab111101; RRID:AB_10859466 |
| anti-CD68 antibody |
Bio-Rad |
MCA1957; RRID:AB_322219 |
| anti-beta actin antibody |
GeneTex |
GTX109639; RRID:AB_1949572 |
|
| Bacterial and virus strains |
|
|
Escherichia coli DH5a competent cells |
Zymo Research |
T3007 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Δ9-Tetrahydrocannabinol (THC) |
Sigma-Aldrich |
T-005 |
| Genistein |
Cayman Chemicals |
10005167 |
| Matrigel® Matrix Basement Membrane Growth Factor Reduced |
Corning |
356231 |
| Matrigel® Basement Membrane Matrix |
Corning |
354234 |
| Y-27632 2HCl (ROCK Inhibitor) |
Selleck Chemicals |
S1049 |
| CHIR-99021 (CT99021) HCl 5mg |
Selleck Chemicals |
S2924 |
| IWR-1 |
Selleck Chemicals |
S7086 |
| B-27 Supplement, minus insulin |
Thermo Fisher Scientific |
A1895601 |
| B-27 Supplement (50x), serum free |
Thermo Fisher Scientific |
17504044 |
| Recombinant Human FGF-2 |
PeproTech |
100-18B |
| Recombinant Human VEGF |
R&D Systems |
293-VE-010/CF |
| EGM-2 Endothelial Cell Growth Medium-2 Bullet Kit |
Lonza |
CC-3162 |
| Doxycycline |
Sigma-Aldrich |
D3072 |
| Polyethylenimine HCl MAX |
Polysciences |
24765-1 |
| Polybrene |
Santa Cruz Biotechnology |
sc-134220 |
| Puromycin |
Cayman Chemical Company |
13884 |
| Oil Red O |
Sigma-Aldrich |
O0625 |
| BstXI |
Thermo Fisher Scientific |
FD1024 |
| BlpI |
NEB |
R0585S |
| DNA ligation kit |
Takara Bio |
6023 |
| Lipofectamine 2000 |
Thermo Fisher Scientific |
11668019 |
| CellTiter-Glo 2.0 Assay |
Promega |
G9241 |
| ROS-Glo™ H2O2 Assay |
Promega |
G8820 |
| GTPase-Glo™ Assay |
Promega |
V7681 |
| Cell ROX™ Deep Red Reagent, for oxidative stress detection |
Thermo Fisher Scientific |
C10422 |
| Calcein-AM |
R&D Systems |
4892-010-01 |
| TRIzol™ Reagent |
Thermo Fisher Scientific |
15596026 |
| RIPA buffer |
Thermo Fisher Scientific |
89901 |
| NuPAGE 4-12% Mini protein gels |
Thermo Fisher Scientific |
NP0321BOX |
| TaqMan™ Universal PCR Master Mix |
Thermo Fisher Scientific |
4305719 |
| TaqMan™ Fast Advanced Master Mix |
Thermo Fisher Scientific |
4444557 |
|
| Deposited data |
|
| Olink and Luminex (https://data.mendeley.com/datasets/s6j8h9yy57/1) |
Mendeley Data |
(https://doi.org/10.17632/s6j8h9yy57.1) |
| Bulk RNA sequencing of EHTs |
NCBI |
GSE198578 |
|
| Experimental models: Cell lines |
|
| Human iPSC Line 1 |
Stanford Cardiovascular Institute (SCVI) Biobank |
SCVI15 |
| Human iPSC Line 2 |
SCVI Biobank |
SCVI113 |
| Human iPSC Line 3 |
SCVI Biobank |
SCVI273 |
| Human iPSC Line 4 |
SCVI Biobank |
SCVI274 |
| CRISPRi iPSC line |
Mandegar et al. 2016
|
Gen1C and Gen2C |
| Human coronary artery endothelial cells (HCAEC) |
The American Type Culture Collection (ATCC) |
PCS-100-020 |
| Human umbilical vein endothelial cells (HUVEC) |
ATCC |
CRL-1730 |
| Human cardiac fibroblasts-ventricular (NHCF-V) |
Lonza |
CC-2904 |
| Human erythroleukemia cells (HEL92.1.7) |
ATCC |
TIB-180 |
| Human neuroblastoma cells (SK-N-FI) |
ATCC |
CRL-2142 |
| U-937 monocytes |
ATCC |
CRL-1593.2 |
| H7 Human embryonic stem cells |
Wicell |
WA07 (H7) |
| HEK293T packaging cells |
Takara Bio |
632180 Lenti-X™ 293T Cell Line |
| CB1-expressing Chem-1 cells |
EMD Millipore |
HTS019RTA |
|
| Experimental models: Organisms/strains |
|
| Mouse: wild type |
The Jackson Laboratory |
C57BL/6J |
| Mouse: LDLR knockout |
The Jackson Laboratory |
B6.129S7-Ldlr<tm1Her>/J |
| Mouse: C57BL/6-Apoe em1Narl/Narl (Apoe knockout mice) |
National Laboratory Animal Center (Taiwan) |
RMRC13302 |
| Mouse: BALB/cAnN.Cg-Foxn1nu/CrlNarl (Nude mice) |
National Laboratory Animal Center (Taiwan) |
RMRC12005 |
|
| Oligonucleotides |
|
| Protospacer sequences of sgRNAs: GGACTAAGCGCAAGCACCTA (control sgRNA); GACAGCGCGAGCGGAGGGAG (CB1 sgRNA#1); GCGCGGGAGACAAGAAGAGG (CB1 sgRNA#2) |
Addgene #83969 |
N/A |
| ON-TARGET plus Human CNR1 (1268) siRNA-SmartPool: GCGAGAAACUGCAAUCUGU (CB1 siRNA#1); GACCAUAGCCAUUGUGAUC (CB1 siRNA#2); GGACAUAGAGUGUGUUUCAUG (CB1 siRNA#3); CAAGAGCACGGUCAAGAUU (CB1 siRNA#4) |
Dharmacon |
L-004711-00-0005 |
| ON-TARGETplus Non-targeting Control Pool |
Dharmacon |
D-001810-10-20 |
|
| Recombinant DNA |
|
| sgRNA backbone plasmid |
Addgene |
#60955 |
| psPAX2 (Lentiviral packaging plasmid) |
Addgene |
#12260 |
| pMD2.G (VSV-G envelope expressing plasmid) |
Addgene |
#12259 |
|
| Software and algorithms |
|
| ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
| R version 3.5.3 |
The R Project |
https://www.r-project.org/
|
| BROOD version 3.0 |
Open Eye Scientific Software |
N/A |
| ROCS version 2.3 |
Open Eye Scientific Software |
N/A |
|
| Other |
|
| High fat diet (40% fat, 1.25% cholesterol) |
Research Diets, Inc |
D12079B, modified |
| Osmotic pumps |
Alzet |
Model 2006 |
| Feeding needle |
Fine Science Tools |
18065-20 |
| Non-invasive blood pressure system |
Kent Scientific Corporation |
CODA-HT4 |
| Silicone rack |
EHT Technologies |
C0001 |
| Teflon spacer |
EHT Technologies |
C0002 |
| Measurement of lipid profiles in mice |
Stanford Animal Diagnostic Lab |
N/A |
| SWEETLEAD |
SWEETLEAD Database (Stanford University) |
N/A |
| UK Biobank |
UK Biobank |
N/A |
