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. Author manuscript; available in PMC: 2022 Aug 24.
Published in final edited form as: Cell Rep. 2022 Jun 28;39(13):111003. doi: 10.1016/j.celrep.2022.111003

Figure 4. Rod OFF pathways drive scotopic image formation but do not drive the scotopic PLR.

Figure 4.

(A) The average pupil constriction over time in response to 1-lux light (475 R*/rod/s) beginning at t = 0 s (dashed line) for control (blue, n = 7) and mGluR6; Opn4 dKO (dark red, n = 7) mice. Shaded outlines represent SEM. All pupil sizes are normalized to the dark-adapted pupil size (before t = 0).

(B) The minimum pupil area (maximal constriction) in response to 1-lux light from t = 0 to t = 30 s. Individuals and SEM are shown. Pupil constriction in littermate control mice and mGluR6; Opn4 dKO mice is significantly different (Student’s t test, p = 2E–5).

(C) Schematic depicting the visually guided behavioral task. Mice were dark adapted for 3 h before experiments, and the testing chamber was kept dark except for the scotopic light stimulus (200 R*/rod/s).

(D) Control mice (blue, n = 5) correctly detect the light stimulus in ~90% of the trials. mGluR6; Opn4 dKO mice (dark red, n = 5) correctly detect the light stimulus comparable with control mice under scotopic conditions (Student’s t test, p = 0.51). Error bars indicate SEM.