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LC‐MS chromatogram showing the 2‐methylglutamate MRM trace in acid‐hydrolyzed QS‐28 peptides. In the presence of QCMT, a new peak was identified as 2‐methylglutamate as confirmed by spiking with a synthetic standard and corresponding retention time changes (see Figure S8). Note that under acid hydrolysis glutamine is converted to glutamate.
