Figure 2.

Alkylating agents differ widely in their ability to conserve protein persulfides. A–C) Whole protein mass spectra of Trx1CS (13.5 μM) persulfidated with Na₂S₂ (300 μM) and alkylated with A) HPE‐IAM, B) IAA, or C) NEM (1 mM each). Peaks corresponding to unmodified or thiol‐alkylated proteins are highlighted in blue and peaks corresponding to per‐/polysulfide‐alkylated proteins are highlighted in yellow. D) Relative proportion of alkylated persulfide recovered with different alkylating agents (n=2–5, error bars represent SD).