Figure 2.
Biocompatibility and cellulose‐dissolving ability of ZIL. A) Chemical structures of ZIL, IL‐1, and IL‐2. B) Viability of A. thaliana seedlings pretreated for 3 h with various concentrations of ZIL, IL‐1, or IL‐2, followed by 24 h of incubation. The viability was determined by the Evans blue assay. Data from four biological replicates are represented as the mean±standard error values. Statistical significance: * P<0.05, ** P<0.01 based on Dunnett's T3 test (n=4). C) 1D WAXD profiles obtained from cellulose microcrystals 2 h after pretreatment with 0, 200, or 400 mM ZIL. D) AFM height images of cellulose microcrystals pretreated with 0, 200, or 400 mM ZIL for 2 h on a highly oriented pyrolytic graphite (HOPG) substrate. Scale bars, 500 nm. Color bars represent the height of the cellulose microcrystal.