FIGURE 6.

IFN-γ suppressed 4-aminopyridine (4-AP)-induced neuronal activity. (A) Experimental sketch: the grid represents the placement of the microelectrode array (MEA) (left). All accounts of one event, detected by a spike sorting algorithm, plotted on top of each other (gray) and the template they were matched to (black, right). All displayed events were recorded from the electrode marked with X in the figure on the left. (B) IFN-γ-curbed network activity in acute neocortical slices. Sample traces (left) recorded from the same electrode (marked with X in the heatmap below) before (black) and under (green) IFN-γ application. (C) Continuous iNOS inhibition via 1400W (10 μM) partly prevented IFN-γ-mediated network activity suppression. (D) IFN-γ required functioning GABA_A receptors to curb network activity. Under continuous bicuculline methiodide (BMI, 10 μM) application, IFN-γ failed to significantly alter event count. Example traces on top and exemplary heatmap below. (E) Relative event count change upon IFN-γ application in the absence of blockers and with 1400W or BMI, respectively. Most events considerably decreased in frequency upon IFN-γ. This attenuation is partly prevented in presence of 1400W and absent in the presence of BMI. Top, middle, and bottom lines of boxes represent percentiles 75, 50, and 25, respectively, and long bars represent means. *p < 0.05.