Fig 6. Overexpression of CD146 suppresses Ang Ⅱ–and TGF–β1–induced EndMT in HUVECs.

A HUVECs were infected with Ad–EV and Ad–CD146 for 48 h. The protein level of CD146 was measured by Western blot analysis. GAPDH served as an internal control. HUVECs were infected with Ad–EV or Ad–CD146 for 48h followed by Ang Ⅱ (1×10⁻⁶ mol/L) (B) or TGF–β1 (10 ng/ml) (C) treatment for 48h. The protein levels of CD146, VE–Cadherin, α–SMA, FSP–1, and Snail were measured by Western blot analysis. GAPDH served as an internal control. *P<0.05, **P<0.01 vs. Ad–EV, ^#P<0.05, ^## P<0.01 vs. Ad–EV+ Ang Ⅱ or TGF–β1. ^&P<0.05, ^&&P<0.01 vs. Ad–EV. Ad–EV: Ad–empty vector.