Fig. 3. Assessment of dynamic changes in immune-related gene expressions in spheroids.

(A) Graphical illustration of spheroid culture, treatment, and assessment. Non–RAP-MP and HS100 spheroids were cultured in MEM-α growth medium with or without cytokine cocktail (IFN-γ and TNF-α) supplementation. Samples were collected and processed for qRT-PCR analysis after culture for 1 to 3 days. (B) Heatmap depiction of gene expression levels. Results are expressed as log₁₀ fold changes versus day 0 (n = 3 independent experiments).