Fig. 4. RBM46-deficient spermatocytes fail to repress mitotic program.

(A) Testis sections from P12 WT and Neurog-cre Rbm46 KO were immunolabeled with pHH3 (phosphorylated histone H3 at Ser¹⁰) and SYCP3, a component of the synaptonemal complex. Aberrant metaphase-like cells with pHH3 signal are indicated by white arrows. Scale bar, 20 μm. (B) Immunofluorescence analysis with pHH3 and SYCP3 antibodies were performed in Neurog-cre Rbm46 KO at P18. pHH3-positive signals were observed in preleptotene- and zygotene-like cells in Neurog-cre Rbm46 KO. (C) Frozen sections from P12 WT and Neurog-cre Rbm46 KO testes were immunostained with α-tubulin to visualize the spindle (green). Monopolar spindles were indicated by arrows. Scale bar, 20 μm. (D) Immunofluorescence of SYCP3 and CCNA2 on testis sections prepared from P12 WT and Neurog-cre Rbm46 KO testis. SYCP3-positive zygotene-like cells that express CCNA2 are indicated by arrows. Scale bar, 20 μm.