Fig. 5.

CADosome-mediated delivery of Cre-recombinase encoding mRNA (Cre-mRNA) in a HeLa reporter cell line. (A) Schematic illustration of HeLa reporter cells shifting from DsRed+ to eGFP+ after Cre-recombinase mediated elimination of the DsRed stop-codon following successful delivery of Cre-encoding mRNA via CADosomes. Created with BioRender.com (B) Percentage eGFP+ cells as analyzed via flow cytometry 24 h after transfection with NT-DOPE CADosomes N/P 9–12 and DOTAP-DOPE N/P 2. Data are represented as mean ± the standard error of the mean (SEM) for minimum three independent repeats (n ≥ 3). Statistical analysis was performed using One Way Anova with Tukey Correction (**** p ≤ 0.0001). (C) Representative flow cytometry dot-plots of non-treated cells (NTC) and NT-DOPE CADosomes N/P 9 or DOTAP-DOPE N/P 2 transfected HeLa reporter cells, respectively.