Fig. 7.

In vivo evaluation of eGFP-mRNA delivery and corneal toxicity of mRNA CADosomes in a rabbit model. (A) Color fundus and fluorescence images of rabbit eyes treated with eGFP-mRNA NT-DOPE N/P 9 CADosomes at a final concentration of 2 μg/mL. The marked yellow circles indicate regions with high eGFP expression at different timepoints post treatment. (B–C) 2D Optical Coherence Tomography (OCT) images visualizing the corneal thickness pre- and post-administration. No significant change could be observed (n = 12 measurements at different locations of the cornea, analyzed via ImageJ software). Scale bars correspond to 100 μm. Statistical analysis was performed using One Way Anova with Tukey Correction (ns p ≥ 0.05). (D) Histology of the corneal surface treated with free eGFP-mRNA and eGFP-mRNA CADosomes, respectively. The presented images are representative of three rabbits for each group (n = 3). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)