TABLE 2.

Plasmids used in this study

Plasmid	Description	Reference or source
pBK8	3.5-kb NsiI fragment from R100-1 (traN-traF) in pBS/SK+; Apr	40
pKAF2	3.0-kb fragment from F (traK-traP) in BamHI-EcoRI sites of pUC118; Apr	This work
pKAF3	0.88-kb fragment from F (traX) in SmaI-HindIII sites of pT7-3; Apr	This work
pKAR1	11.6-kb EcoRI fragment from R100-1 (traK-trbC) in pT7.4; Apr	This work
pKAR2	3.0-kb fragment from R100-1 (traK-traP) in BamHI-EcoRI sites of pUC118; Apr	This work
pKAR3	2.4-kb BamHI-PstI fragment from R100-1 (traY-traK) in pACYC177; Kmr	This work
pKAR4	2.1-kb SmaI-SacI fragment from R100-1 (trbE-trbA) in SmaI site of pACYC177; Apr	This work
pKAR5	5.1-kb SacI-EcoRI fragment from R100-1 (traQ-traH) in SmaI-HindIII sites of pACYC177; Apr	This work
pKAR6	3.0-kb fragment from R100-1 (traG) in BamHI-HindIII sites of pT7.3; Apr	This work
pKAR7	0.82-kb fragment from R100-1 (traX) in PstI-HindIII sites of pT7-3; Apr	This work
pKI164	2.6-kb HincII fragment from F (traP-traR) in pACYC177; Apr	57
pKI175	6.2-kb AvaI fragment from F (traW-traQ) in pACYC177; Apr	85
pKI375	3.0-kb Asp7001 fragment carrying F (trbC-traF) genes; pBS/SK+; Apr	47
pLF164	1.4-kb PstI fragment of F (traA-traE) in pUC8, Apr	This work
pLF107	1.5-kb SmaI fragment of R100-1 (traA-traE) in pUC8, Apr	This work
pRS29	EcoRI f1 and f15 fragments from F (traV-traH) in pSC101; Tcr	72
pRS27	EcoRI f6 and f15 fragments from F (traM-traR) in pSC101; Tcr	72
pRS31	EcoRI f2, f17, and f19 fragments from F (traS-finO::IS3) in pSC101; Tcr	72
pRS1670	F traG in pGEX-1 vector; Apr	25
pBluescript SK(+)	ColE1 ori; Apr	Stratagene
pACYC177	p15A ori; Apr Kmr	11
pT7-3, pT7-4	T7 promoter; ColE1 ori; Apr	74
pSC101	Tcr	15
pOX38-Km	pOX38-Km Tra+ RepFIA+ Kmr f1 HindIII fragment of F plus HindIII fragment of Tn5	10
R100-1	R100-1 Tra+ Cmr Far Smr Spr Sur Tcr	4